宫颈病变组织中P53、Ki-67、CD34的表达及临床意义

摘要

目的：探讨宫颈病变组织中P53、Ki-67、CD34的表达及其临床意义。方法应用免疫组化SP法测定正常宫颈上皮、宫颈上皮内瘤变和宫颈浸润癌组织中P53、Ki-67、CD34的表达。结果 P53在正常宫颈上皮、宫颈上皮内瘤变和宫颈浸润癌组织中的阳性表达率分别为0％（0/28）、39.58％（19/48）和69.44％（25/36），Ki-67的阳性率分别为3.57%（1/28）、52.08％（25/48）和86.11％（31/36），CD34标记的MVD值分别为（12.46±4.63）/HP、（30.12±7.46）/HP和（42.12±5.48）/HP，P53、Ki-67、CD34在三种宫颈病变中的阳性表达率两两比较，差异均有统计学意义。结论 P53、Ki-67、CD34表达强度均随宫颈病变的进展而增加，P53和Ki-67的表达与浸润癌的分化程度无关，CD34的表达与浸润癌的分化程度正相关。检测P53、Ki-67和CD34的表达对CIN癌变的早期诊断及其分层治疗有一定的指导意义。%Objective To investigate the expressions and clinical significances of p53, ki-67 and cd34 in the cervical lesions. Methods Immunohistochemica1 method was used to detect the expressions of P53, Ki-67 and CD34 in the normal uterine cervix, cervical intraepithelial neoplasia(CIN) and cervical infiltrating carcinoma. Results In normal uterine cervix, CIN and cervical in-filtrating carcinoma, the positive expression rates of P53 were respectively 0%(0/28), 39.58%(19/48) and 69.44%(25/36), and the positive expression rates of Ki-67 were respectively 3.57%(1/28), 52.08%(25/48) and 86.11%(31/36). The microvessel density (MVD) of marked CD34 was respectively (12.46±4.63)/HP, (30.12±7.46)/HP and (42.12±5.48)/HP in the above mentioned tissues. The pair-wise comparison showed that the positive expression rates of P53, Ki-67 and CD34 had statistical difference in the CIN I-II, CIN III and cervical infiltrating carcinoma tissues(P<0.05). Conclusion The expressions of P53, Ki-67 and CD34 increased with the progression of the cervical lesions. The expression of P53 and Ki-67 had no correlation with the differentiation degree of infiltrat-ing carcinoma, while the expression of CD34 was positively correlated with the differentiation degree of cervical infiltrating carci-noma. Combined detection of P53, Ki-67 and CD34 can be helpful for the early diagnosis and classification of CIN.