不同高压氧预处理对大鼠肾缺血再灌注损伤的研究

摘要

Aim To investigate effects of different programs of preconditioning with hyperbaric oxygen( HBO ) on renal ischemia-reperfusion injury in rats. Methods Fifty SD rats were divided into five groups randomly : Group S : sham operation ( n = 10 ); Group I/R : rats l/R injury( n = 10 ); (;roup Hl-3 : rats were given HBO pretreatment 24 h before renal I/R for 4, 8 and 16 consecutive days respectively. In group I/R, rat model of renal ischemia-reperfusion injury ( IRI ) was established ( bilateral renal artery clamping, after 45 minutes. the clamps were removed ). During the 24-hour reperfusion period, the rat kidneys were observed for restoration of blood flow returning to their original color. In group S, Sham surgery consisted of the same surgical procedure except that clamps were not applied.Group H1-3 : rat was pretreated with HBO for 4. 8 and 16 days respectively, followed by the same surgical procedure. The animals were then killed and serum maleic dialdehyde( MDA ) . blood urea nitrogen( BUN ). serum creatinine( Scr ) were examined. Renal histology in I/R ARF was observed under an optical microscope. The expression of TNF-α and IL-6 were detected by ELISA. Results BUN, Cr,MDA, TNF-α . IL-6 in IR group were higher than those in S group ( P ＜0. 01 ). BUN, Cr, MDA. TNF-α. IL-6 in H1 , H2, H3 group were lower than those in the IR group ( P ＜ 0. 05 ). TNF-α . IL-6 . MDA higher than the H2 group ( P ＜ 0. 05 ). Preconditioning with 4 , 8 or 16 d of HBO attenuate the I/R ARF in kidney morphology and function. and decreased the TNF-α and IL-6 expression. Conclusion Preconditioning with hyperharic oxygen attenuates rat renal ischemia-reperfusion injury.%目的 研究不同时间高压氧预处理对大鼠肾脏缺血再灌注损伤的影响并探讨其机制.方法 雌性SD大鼠50只随机分为5组:假手术组(S组,n=10)打开腹腔不夹闭肾蒂;缺血再灌注组(IR组,n=10)夹闭双侧肾蒂30 min后再灌注;高压氧预处理1组(H1,n=10); 高压氧预处理2组(H2,n=10);高压氧预处理3组(H3,n=10),分别接受连续高压氧4、8、16 d治疗.高压氧结束后24 h,行肾脏缺血再灌注手术.每组大鼠术后24 h后处死,检测血尿素氮(BUN)、肌酐(Cr)、丙二醛(malonic dialdehyde,MDA),肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin- 6,IL- 6)及肾脏病理检查.结果 IR组BUN、Cr、MDA、TNF-α、IL- 6高于S组(P<0.01),IR组肾小管上皮细胞均有不同程度的坏死,间质水肿,大量炎细胞浸润,血管渗透性增加,肾小球萎缩;H1、H2、H3组BUN、Cr、MDA、TNF-α、IL- 6均低于IR组(P<0.05),H1、H2、H3组肾脏仅有肾小管上皮细胞肿胀,少量炎症细胞浸润;H3组TNF-α、IL- 6、MDA高于H2组(P<0.05).结论 连续高压氧4、8和16 d预处理均能减轻大鼠肾脏缺血再灌注损伤;连续16 d高压氧预处理作用较8 d作用减弱.