HPLC-DAD法检测女宝胶囊中的微量金橙Ⅱ

摘要

目的 建立女宝胶囊中微量金橙Ⅱ的测定方法,了解女宝胶囊中金橙Ⅱ的分布状况.方法 女宝胶囊中的金橙Ⅱ经提取后先用薄层色谱定性再用反相高效液相色谱-二极管阵列检测器(DAD)检测[1].结果 部分厂家部分批次的女宝胶囊中检测出含有金橙Ⅱ.结论 某些药厂在原料药采购环节和原药材检验环节还存在一定问题,有关部门应加以监督.%Aim To establish a method for the determination of the trace orange Ⅱ in nvbao capsule and to understand its distribution in nvbao capsule. Methods The orange Ⅱ in nvbao capsule was tested by thin layer chromatography( TLC )and reversed-phase high-performance liquid-photodiode array detector( RP-HPLC-DAD )method, respectively. Results Trace orange Ⅱ in some batches of nvbao capsule was detected. Conclusion Some problems exist in the procurement and inspection of crude drug,on which the related departments should supervise.