N-乙酰半胱氨酸对急性乙醇暴露致小鼠睾丸损伤的保护作用

摘要

目的：在成功建立乙醇诱导小鼠睾丸损伤模型的基础上，该研究主要探讨N-乙酰半胱氨酸( NAC)对急性乙醇暴露致小鼠睾丸损伤的保护作用。方法该研究由2个实验组成。实验1：28只雄性小鼠被随机分成4组，对照组和乙醇(1、3、6 g· kg-1)处理组；实验2：24只雄性小鼠被随机分成4组，对照组、乙醇组、NAC组和NAC+乙醇组。2个实验均于乙醇处理后24 h剖杀小鼠，取睾丸称重，各组小鼠睾丸用MDF液固定，制作石蜡切片，以备后续睾丸HE染色和免疫组织化学检测。结果3和6 g·kg-1乙醇暴露明显引起小鼠睾丸内多核巨细胞并向管腔内生殖细胞脱落等组织病理学改变；1和3 g·kg-1乙醇暴露明显抑制小鼠睾丸生殖细胞增殖；NAC明显保护3 g·kg-1乙醇暴露所致小鼠睾丸病理学损伤，显著减轻3 g·kg-1乙醇暴露对小鼠睾丸细胞增殖的抑制作用。结论 NAC明显保护乙醇急性暴露对小鼠睾丸组织病理学损伤。%Objective To investigate the protective role of N-acetylcysteine on acute ethanol-induced testicular damage,based on an established mouse model of ethanol-induced testicular injury. Methods The current study included two separate experiments. Experi-ment 1,twenty-eight male mice were assigned into four groups,control group and three ethanol-treated groups. All male mice except for controls received ethanol (1,3 and 6 g·kg-1,i. g. ) administration. Experiment 2,twenty-four male mice were assigned into four groups,control,NAC,ethanol,and NAC+ethanol groups. Male mice in NAC +ethanol group were pretreated with NAC ( 100 mg · kg-1 ,i. p. ) at 12 h and 0. 5 h before ethanol (3 g·kg-1 ,i. g. ) and then received a dose of NAC (100 mg·kg-1 ,i. p. ) at 12 h af-ter ethanol treatment. Other mice except for control (either saline) and NAC group were injected with ethanol (3 g·kg-1,i. g. ). All male mice were sacrificed at 24 h after ethanol treatment. Mouse testes were weighed and collected for HE staining and immunohisto-chemical detection. Results Acute ethanol (3,6 g·kg-1 ) exposure markedly caused multinucleated giant cells and sloughing of germ cells in testicular seminiferous tubules. Acute ethanol (1,3 g·kg-1 ) exposure obviously inhibited germ cell proliferation in mouse testes. NAC protects against acute ethanol-induced testicular histopathological damages and inhibition of germ cell proliferation. Conclu-sions N-acetylcysteine protects against acute ethanol-induced testicular histopathological damage in mouse.