斑蝥素、斑蝥酸钠和斑蝥酸镁抑制人胃癌 SGC －7901细胞增殖的研究

摘要

目的：比较斑蝥素、斑蝥素酸钠和斑蝥素酸镁对人胃癌 SGC －7901细胞增殖的影响，筛选出最佳斑蝥素类抗肿瘤药物。方法将斑蝥素、斑蝥素酸钠及斑蝥素酸镁作用于人胃癌细胞 SGC －7901；采用 CCK －8法检测上述三种药物对 SGC －7901细胞的生长抑制率。结果斑蝥素、斑蝥素酸钠和斑蝥素酸镁均对 SGC －7901细胞有抑制作用，且呈效应－剂量关系。上述三种药物的半数抑制浓度分别为18．591、17．676、4．105μmol·L －1。结论斑蝥素、斑蝥素酸钠和斑蝥素酸镁均能抑制人胃癌细胞生长，其中斑蝥素酸镁的抗肿瘤活性最佳。%Objective To compare the inhibitory effects of cantharidin,cantharidin sodium and cantharidin magnesium on human gastric cancer cell (SGC -7901)proliferation,and to screen out the most efficacious cantharidin antineoplastic drug.Methods To detect the inhibitory rate of the proliferation of SGC -7901 cells of cantharidin,cantharidin sodium and cantharidin magnesium by CCK -8 assay after the three drugs were applied to these cells.Results Cantharidin,cantharidin sodium and cantharidin magnesium have inhibitory effect on SGC -7901 cells,and showed a dose -response relationship.The median inhibitory concentrations of these three drugs were 18.591,17.676,4.105 μmol·L -1 respectively.Conclusion Cantharidin,cantharidin sodium and cantharidin magnesium can inhibit human gastric cancer SGC -7901 cell growth,and the antitumor activity of cantharidin magnesium ranks the best.