抑郁症患者外周血中大麻素受体1和脂肪酸酰胺水解酶的mRNA表达水平及意义

摘要

目的 探讨抑郁症患者外周血中大麻素受体1(CB1)和脂肪酸酰胺水解酶(FAAH)的mRNA表达水平及意义.方法 选择43例未经治疗的抑郁症患者(抑郁组)和43例健康志愿者(健康组),采用实时荧光定量反转录聚合酶链式反应(RT-PCR)技术检测两组患者外周血中CB1和FAAH的mRNA表达水平,观察抑郁症患者与健康人群的CB1和FAAH水平变化;采用汉密尔顿抑郁量表(HAMD)和Stroop色词测试对抑郁症患者的抑郁程度和认知功能进行评估,分析CB1和FAAH表达水平与测试结果的相关性.结果 (1)抑郁症患者的CB1-mRNA、FAAH-mRNA表达水平均明显低于健康组(P＜0.05);(2)抑郁症患者的CB1表达水平与HAMD中的体质量变化因子正相关(r=1.021,P=0.001);FAAH表达水平与HAMD中的认知障碍因子负相关(r=-0.065,P=0.023);(3)抑郁症患者FAAH-mRNA表达水平影响患者的注意力和反应能力(P＜0.05).结论 CB1受体可以影响抑郁症患者的能量代谢,FAAH会对抑郁症患者的认知障碍产生影响,提示抑郁症患者外周血CB1-mRNA、FAAH-mRNA表达量与抑郁障碍具有相关性.%Objective To study the peripheral blood of patients with depression cannabinoid receptor 1 (CB1) and fatty acid amide hydrolase (FAAH) mRNA expression levels and significance.Methods The peripheral blood CB1 and FAAH mRNA expression level of two groups of patients:43 patients with untreated depression (depression group) and 43 cases of healthy volunteers (healthy group) were detected byreal-time fluorescent quantitative (rt-PCR)technology,which were compared with healthy people.Using the Hamilton depression rating scale (HAMD) and the Stroopcolour word test to evaluate the degree of depression in patients with depression and cognitive function assessment,by which to analysis expression level of CB1 and FAAH and the correlation of test results.Resuits The expression levels of CB1-mRNA and FAAH-mRNA in patients with depression were significantly lower than the control group (P ＜ 0.05).The changes of body weight factor in patients with depression the expression level of CB1 and HAMD in positive correlation (r =1.021,P =0.001) while cognitive impairment factor FAAH expression level and HAMD in negative correlation (r =-0.065,P =0.023).The expression level of FAAH-mRNA of patients with depression can influence patient's attention and response ability (P ＜ 0.05).Conclusions CB1 receptor can affect energy metabolism in patients with depression andFAAH will have an impact on cognitive impairment in patients with depression,which suggesting that peripheral blood CB1-mRNA,FAAH-mRNA expression in patients with depression and depressive disorder associated with positive correlation.