骨髓间充质干细胞复合I型胶原修饰的聚乳酸聚乙醇酸对骨质疏松大鼠骨缺损的影响

摘要

Objective To study the effects of PLGA/CoI microspheres combined with BMSCs on bone defects in animal model with osteoporosis.Methods Thirty Sprague Dawley rats were ovariectomized to establish osteoporosis animal models,and the intertrochanteric bone defect was made with electric drill.The models were randomly assigned into sham group (treated with sham operation),control group (the intertrochanteric bone defect sites were treated with phosphate buffer saline) and drug administration group (the intertrochanteric bone defect sites were treated with PLGA/CoI microspheres combined with BMSCs).The bone mineral content,bone mineral density,trabecular thickness,percentage of trabecular area and trabecular spacing after operaton for 1,3 months were compared.Results After 7 days of coculture,BMSCs seeded on PLGA/CoI microspheres had nice adherence and proliferation.The bone mineral density,trabecular thickness and percentage of trabecular area were larger,the trabecular spacing value was smaller in drug administration group compared with the control group after operation for one month (all P<0.05),respectively;the change of bone mineral content was insignificant (P>0.05).Compared with the control group,the bone mineral content,bone mineral density,trabecular thickness and percentage of trabecular area increased significantly in drug administration group (all P<0.05);the trabecular spacing value decreased significantly in drug administration group (P<0.05).Conclusions The bone defect of osteoporotic site can be repaired,the trabecular reconstruction and bone quality of osteoporotic site also can be improved after the injection of the PLGA/CoI microspheres combined with BMSCs.%目的 通过将骨髓间充质干细胞复合I型胶原修饰的聚乳酸聚乙醇酸(PLGA)微球支架注入骨质疏松大鼠股骨转子间,探讨其对骨缺损后骨质量的局部改善情况.方法 通过切除30只SD雌性大鼠双侧卵巢建立骨质疏松动物模型,并用电钻在股骨转子间人为制作骨缺损;实验动物按随机数字表法均分为给药组、模型组和假手术组.给药组大鼠骨缺损部位注入骨髓间充质干细胞复合I型胶原修饰的PLGA微球,模型组大鼠骨缺损部位注入PBS缓冲液,假手术组大鼠行假手术.分别考察术后1、3个月骨缺损部位的骨矿含量、骨密度、骨小梁厚度、骨小梁面积百分比及分离度.结果 骨髓间充质干细胞与I型胶原修饰的PLGA微球支架体外培养7 d,发现细胞在支架表面黏附、生长良好.与模型组比较,给药组动物术后1个月的骨密度、骨小梁厚度、骨小梁面积百分比均显著增大,骨小梁分离度显著减小,均差异有统计学意义(P<0.05),骨矿含量变化不明显(P>0.05);给药组动物术后3个月的骨矿含量、骨密度、骨小梁厚度、骨小梁面积百分比均显著大于模型组,骨小梁分离度显著小于模型组,均差异有统计学意义(P<0.05).结论 骨髓间充质干细胞复合I型胶原修饰的PLGA微球可修复大鼠骨质疏松部位骨缺损,改建骨小梁结构,提高骨质量.