利用微卫星DNA标记分析贵州3个地方猪种的遗传多样性

摘要

[Objective] The research aimed to provide the genetic basis for the protection, development and utilization of Guizhou local pig breeds. [Method] From 27 pairs of porcine microsatellite primers recommended by Food and Agriculture Organization of the United Nations (FAO) and International Society for Animal Genetics (ISAG), six pairs (S0155, SW240, IGF1, SW951, SW857, SW24) were selected for microsatellite DNA detection of three Guizhou local pig breeds, including Nuogu Pig, Kele Pig and Guanling Pig. Subsequently, their genetic diversities were analyzed. [Result] The three pig breeds were high polymorphic at the six microsatellite loci (PIC＞0.5). The Neis standard genetic distance of them was 0.206 3-0.481 5. The genetic distance between Nuogu Pig and Kele Pig was the closest, and that between Nuogu Pig and Guanling Pig was the furthest. [Conclusion] The three Guizhou local pig breeds are in high genetic diversities. Nuogu Pig is a special type of Kele Pig, an excellent Chinese local pig breed.