多壁碳纳米管负载Fe3O4磁性纳米粒子表面吸附增强过氧化酶的催化活性

摘要

Fe3O4 magnetic nanoparticles were prepared by co-precipitation and supported on the surface of aminopyridine-grafted multiwalled carbon nanotubes (MWCNT-AP), affording a superparamagnetic Fe3CVMWCNT-AP composite with good dispersity. The FeaCVMWCNT-AP composites were characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and hysteresis curve measurements. Transmission electron microscopy (TEM) experimental results suggest that the Fe3O4 nanoparticles are mainly adsorbed on the tips of the MWCNT-AP. UV-Vis absorption and fluorescence results demonstrate that horseradish peroxidase (HRP) can be physically adsorbed onto the surfaces of the FesC/MWCNT-AP composite. Under acidic conditions (pH 4.0), the maximum reaction rate (v) value of the HRP increases 3-fold after interaction with Fe3Cy MWCNT-AP.%采用共沉淀法制备了Fe3O4磁性纳米粒子,将其负载于氨基吡啶修饰多壁碳纳米管(MWCNT-AP)上,得到具有良好的分散性和超顺磁性的Fe3O4/MWCNT-AP复合物.通过傅里叶变换红外(FT-IR)光谱、X射线衍射(XRD)和磁滞回线测量等方法对Fe3O4/MWCNT-AP复合物进行了表征.扫描电镜(TEM)结果表明:Fe3O4磁性纳米粒子多集中于碳纳米管MWCNT-AP的端部,形成的复合物在极性溶剂中具有良好的分散性和超顺磁性;辣根过氧化酶(HRP)可通过物理作用吸附于Fe3O4/MWCNT-AP复合物表面.酸性条件下(pH 4.0),Fe3O4/MWCNT-AP复合物使HRP的最大反应速率(Vmax)提高了3倍.