卡托普利和依那普利对血管平滑肌细胞内钙的影响

摘要

To determine whether angiotensin-converting enzyme inhibitors can affect Ca2+ handling in cultured aortic smooth muscle cells (ASMC) directly.METHODS: Cultured ASMC derived from rat aorta were loaded with the intracellular Ca2+ ([Ca]i2+)fluorescent indicator Fura 2-AM and digital image processing technique was used. RESULTS: Resting [ Ca2 + ]i was greater in ASMC from SHR vs WKY(P ＜ 0.01 ). KCl-, norepinephrine (NE)-,and angiotensin Ⅱ (Ang)- induced [ Ca2 + ] i increases were enhanced in ASMC of SHR vs WKY (220± 6, 212 ± 8, and 215 ± 14 vs 199 ± 6, 202 ± 7,(Cap) and enalapril (Ena) had no inhibitory effect on KCl-, NE-, and Ang-induced [Ca2+ ]i increases in ASMC of WKY. Cap and Ena inhibited KCl-,NE-, and Ang-increased [Ca2+ ]i in ASMC of SHR tively). Ena and nifedipine similarly decreased KCl-, NE-, and Ang-increased [Ca2+ ]i. CONCLUSION: Cap blocked KCl-, NE-, and Angincreased ([ Ca2+ ]i) via a voltage-dependent Ca2+channel of which function and specificity was altered in ASMC of SHR.%检测ACE抑制剂对主动脉平滑肌细胞内Ca2+的影响.方法:用荧光标计和图象处理技术结果:SHR细胞内Ca2+以及KCl,NE和Ang在SHR细胞引起的Ca2+增加多于WKY细胞.Cap和Ena不影响KCl和Ang在WKY细胞的作用,但Cap,Ena和Nif抑制KCl,NE和Ang在SHR细胞的作用.结论:Cap和Ena阻断功能和特异性已改变的电压依赖性钙通道.