消瘀片对粥样硬化兔血管壁组织中内皮素-1、一氧化氮及细胞凋亡的影响

摘要

AIM: To investigate the mechanism of xiaoyu tablet onreduction of smooth muscle cells (SMC) in atherosclerotic vessel wall. METHODS: The atherosclerotic model was performed in male New Zealand rabbits that were given high fat diet and abrasion of the abdominal aorta endothelial cells. The rabbits were then administered with xiaoyu tablet 0.16-0.32 g@kg-1@d-1 for 16 weeks. Changes in morphology, endothelin (ET)-i,nitric oxide (NO), and apoptotic cells of atherosclerotic vessel wall were determined by the microscopy,radioimmunoassay, colorimetric method, the techniques of DNA in situ end labeling, and image pattern analysis,respectively. RESULTS: After 16 weeks of xiaoyu tablet treatment, intimal thickness and SMC in atherosclerotic vessel wall were diminished, ET-1 was decreased by 8.2 % -42.6 %, NO was increased by 7.5 %- 54.2 %, and labeled apoptotic nuclei were markedly decreased, the area and integral optical density of positive granule were (846 ± 308) μm2 and 3425 ±1374 in atherosclerotic group and (225 ± 60) μm2 and 1445 ± 606 in xiaoyu tablet 0.32 g/kg group,respectively. CONCLUSION: Xiaoyu tablet not only inhibited proliferation of SMC through reducing ET-1 in atherosclerotic vessel wall, but also induced apoptosis of SMC by increasing NO in vessel wall.%目的:探讨消瘀片减少粥样硬化血管壁中平滑肌细胞数的作用机制.方法:雄性新西兰兔高脂饮食加腹主动脉剥脱术制成腹主动脉粥样硬化模型,通过显微镜检查、放免法、比色法、原位末端标记及图象分析技术分别测定连续给予消瘀片0.16-0.32g@kg-1@d-1治疗16周后血管壁组织的形态学、内皮素(ET)-1、一氧化氮(NO)含量及细胞凋亡的变化.结果:消瘀片治疗16周后,粥样硬化血管壁的内膜厚度和平滑肌细胞数明显减少,血管壁组织中的ET-1含量降低8.2%-42.6%,NO含量增加7.5%-54.2%,凋亡细胞阳性反应颗粒明显减少,其所占的面积和积分光密度值在粥样硬化组是(846±308)μm2和3425±1374,消瘀片0.32g/kg组是(225±60)μm2和1445±606.结论:消瘀片通过降低血管壁中的ET-1抑制平滑肌细胞的增生,通过增加血管壁中的NO诱导细胞凋亡.