石见穿酸性多糖SC4的结构特征和生物活性

摘要

AIM:To study the chemical characterization and some biological activities of an acidic polysaccharide,named SC4 from Salvia chinensis.METHODS:Chemical and spectral methods were employed to identify the structural characterization of SC4.Effect of SC4 on the proliferation of T- and B-lymphocytes both in vivo and in vitro was measured at various concentrations,and the protective effects of SC4 on PC12 cells against H2O2-induced injury was observed.RESULTS:SC4 was a highly branched polysaccharide with mean molecular weight of 4.5 × 105,composed of Rha,Xyl,Set,Gal,and GalA in the molar ratio of 1.0:7.0:5.3:1.2:4.2.Methylation analysis and NMR identified the linkages of the residues of SC4.SC4 increased the B-lymphocyte proliferation and spleen weight remarkably while no obvious changes were examined in T-lymphocyte proliferation,thymus weight,and the level of IgG and C3 in blood of mice.Moreover,SC4 enhanced the PC12 cells viability after H2O2 treatment.CONCLU-SION:SC4,the acidic polysaccharide with complicated structure,was a B-lymphocyte stimulator and protected PC12 cells at the concentration of 20 mg/L against H2O2-induced injury.%目的:研究从石见穿(Salvia chinensis)中得到的酸性多糖SC4的化学结构和生物活性.方法:利用化学和光谱方法分析了SC4的结构特征,并观察了SC4对淋巴细胞增殖的影响以及增强PC12细胞对抗H2O2造成的损伤的作用.结果:SC4的分子量为4.5×105,由Rha,Xyl,Ser,Gal和GalA组成,摩尔比为1.0:7.0:5.3:1.2:4.2.甲基化和13C NMR进一步确定了SC4中各糖残基的连接方式.SC4能显著促进B淋巴细胞的增殖,增加小鼠脾重,增强PC12细胞对抗H2O2造成的氧化损伤的能力.结论:SC4为一多分枝的酸性多糖,其免疫活性的靶细胞为B淋巴细胞,并显著增强PC12细胞对抗H2O2造成的损伤的能力.