Salvianolic acid A inhibits angiotensin Ⅱ-induced proliferation of human umbilical vein endothelial cells by attenuating the production of ROS

摘要

To investigate the action of salvianolic acid A (SalA) on angiotensin Ⅱ (Ang Ⅱ)-induced proliferation of human umbilical vein endothelial cells (HUVECs) and the possible signaling pathways mediating this action.Methods:Cell proliferation was examined with MTT assay.The expression levels of Src phosphorylation (phospho-Src),Akt phosphorylation (phospho-Akt),and NADPH oxidase 4 (Nox4) in HUVECs were determined by Western blot.The production of reactive oxygen species (ROS) was estimated using fluorescence-activated cell sorting (FACS).Results:SalA (6.25-50 μmol/L) did not affect the viability of HUVECs.Treatment of HUVECs with Ang Ⅱ(1 μmol/L) markedly increased the cell viability; pretreatment of HUVECs with SalA (12.5,25,and 50 μmol/L) prevented Ang Ⅱ-induced increase of the cell viability in a concentration-dependent manner.Treatment of HUVECs with Ang Ⅱ(1 μmol/L) markedly up-regulated the protein expression levels of phospho-Src,phospho-Akt (473) and Nox4; pretreatment of HUVECs with SalA (12.5,25,and 50 μmol/L) blocked all the effects in a concentration-dependent manner.Treatment of HUVECs with Ang Ⅱ(1 μmol/L) dramatically increased ROS production in HUVECs; pretreatment of HUVECs with SalA (12.5,25,and 50 μmol/L) blocked the ROS production in a concentration-dependent manner.Conclusion:SalA inhibits Ang Ⅱ-induced proliferation of HUVECs via reducing the expression levels of phospho-Src and phospho-Akt (473),thereby attenuating the production of ROS.