MicroRNA-132 suppresses cell proliferation in human breast cancer by directly targeting FOXA1

摘要

Dysregulation of microRNAs (miRNAs) has been implicated in cancer.Recently,miR-132 has been reported to be downregulated in the tissues of patients with breast cancer.In this study,we investigated the functional role of miR-132 and its direct target FOXA1 in breast cancer cells.In 30 human breast cancer tissues,FOXA1 was significantly overexpressed and negatively correlated with miR-132 expression.A bioinformatics analysis suggested that FOXA1 was a potential target of miR-132.Furthermore,dual luciferase reporter assays revealed that miR-132 dose-dependently inhibited the luciferase activity of the wt 3'UTR of FOXA1 rather than the mut 3'UTR of FOXA1 in human MDA-MB-468 and SK-BR3 breast cancer cells.Moreover,ectopic miR-132 expression significantly inhibited FOXA1 protein expression,whereas miR-132 knockdown promoted FOXA1 expression in the breast cancer cells.Ectopic miR-132 expression also suppressed proliferation of the breast cancer cells,whereas miR-132 knockdown promoted proliferation of the breast cancer cells,which was reversed by knockdown of FOXA1 expression.We conclude that MiR-132 suppresses proliferation of breast cancer cells at least partially though inhibition of FOXA1.These results suggest that miR-132 and FOXA1 may be potential biomarkers or therapeutic targets in breast cancer.