应用免疫组化LSAB法及图像分析技术、形态定量检测34例细支气管肺泡癌（BAC）组织中α1-抗胰蛋白酶（α1-AT）的表达，探讨其表达强弱与BAC临床、生物学行为及预后的关系。结果发现：α1-AT阳性细胞胞浆平均吸光度（单位面积内相对含量）男性明显高于女性（P<0.01）；60岁以上组高于60岁以下组（P<0.05）；局部淋巴结转移组显著低于无转移组，术后有远处转移组显著低于无转移组，两组间的表达均具有显著性差异（P<0.05）；1年内短期死亡组显著低于1～3年以上生存组（P<0.01）。结果提示：α1-AT表达水平与BAC转移能力呈负相关，其表达强度越高越不容易形成转移。α1-AT表达可能是BAC预后判断的重要指标之一。%The relationship between the expression of α1-antitrypsin (α1-AT) and the prognosis as well as the biological behavior of bronchoalveolar carcinoma (BAC) was studied. The immunohistochemical expression of α1-AT in tissues of 34 BAC patients was detected. The cellular expression of α1-AT was detected by using LSAB assay and computer-assisted image analysis system (IAS) for morphological and quantitative analysis. Meanwhile, all patients were followed up no less than one year postoperatively. It was found the expression of α1-AT in the male patients was higher than that in the females (P<0.01), that in the patients over 60 years higher than that in those below 60 years (P<0.05) and that in the patients with local nodal metastasis significantly lower than those without metastasis (P<0.01). According to the findings of follow-up, the expression of α-AT in the group with distant metastasis after operation was lower than in the group without distant metastasis (P<0.05). In addition, the expression of α-AT in the patients survived less than one year postoperatively was significantly lower than that in the patients survived only 1-3 years (P<0.01). It was indicated that there might be a negative correlation between the expression of α1-AT and metastasis of BAC and α1-AT might be an important factor to estimate the prognosis of BAC.
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