5-氟尿嘧啶联合奥沙利铂对SW480细胞成瘤性及其Galectin-3表达的影响

摘要

Objective To study the effects of 5 Fu combined with Oxaliplatin on tumorigenesis of SW480 cells and the ex pression of Galectin 3 in the tumor tissues- Methods Human colon cancer xenografts in nude mice were established by using SW480 cells- 5 Fu group,Oxaliplatin group,5 Fu+Oxaliplatin group,and saline control group were set up. By using immuno histochemistry the expression of Galectin 3 was detected in the xenografts- Image signal acquisition and analysis system was ap plied for image scanning analysis- Results The rate of tumor suppressor in 5 Fu group,Oxaliplatin group and 5 Fu+Oxaliplatin group was 30.53%, 34.15% and 67. 22% respectively. Immunohistochemistry revealed that the Galectin 3 expression in 5 Fu+ Oxaliplatin group (28. 875 + 7. 491) was significantly reduced as compared with saline control group (281. 438 + 45. 639) ,5 Fu group(157. 644 + 33. 721)and Oxaliplatin group(150. 682+22. 539) (P<0. 05 or P<0. 01). Conclusion 5 Fu and Oxaliplatin could inhibit the growth of SW480 cells with a synergic effect. The Galectin 3 expression is decreased significantly in 5 Fu+Ox aliplatin group,indicating Galectin 3 protein may play an important role in tumor cells growth.%目的 探讨5-氟尿嘧啶(5-Fu)联合奥沙利铂对SW480细胞成瘤性及其对肿瘤中Galectin-3(半乳糖凝集素-3)表达的影响.方法 以人结肠癌SW480细胞建立裸鼠移植瘤模型,实验分5-Fu组,奥沙利铂组,5-Fu+奥沙利铂组和注射等体积生理盐水的对照组.采用免疫组织化学方法检测移植瘤中Galectin-3的表达,以图像信号采集与分析系统进行图像扫描分析.结果 5-Fu组、奥沙利铂组和5-Fu+奥沙利铂组抑瘤率分别为30.53%、34.15%和67.22%;免疫组化显示给药组肿瘤组织中Galectin-3表达明显减弱,Galectin-3在联合用药组肿瘤组织中表达(28.875±7.491)较对照组(281.438±45.639)和单独用药组(157.644±33.721,150.682±25.539)明显减弱,图像灰度值统计分析显示差异均具有统计学意义(P＜0.05或P＜0.01).结论 5-Fu和奥沙利铂可抑制肿瘤细胞生长,二者联用抑瘤作用增强.肿瘤的生长体积与Galectin-3的表达具有同向性,提示两者之间可能存在直接或间接的联系.