目的：探讨COX-2选择性抑制剂 NS398对食管癌细胞株 EC109迁移能力的影响及其可能机制。方法选用食管癌细胞株 EC109为靶细胞,分别加入不同浓度(0、50、100μmol/L)的 NS398,利用细胞划痕实验,观测细胞48 h内迁移能力变化；采用逆转录PCR方法检测目标基因 COX-2、RhoA、CDC42及 Rac-1的表达变化。结果细胞划痕实验显示,50μmol/L NS398组迁移距离[24 h：(50.81±8.51)μm,48 h：(109.67±18.38)μm]及100μmol/L NS398组迁移距离[24 h：(53.15±9.58)μm,48 h：(97.42±13.20)μm]与对照0μmol/L NS398组迁移距离[24 h：(68.72±5.68)μm,48 h：(129.68±10.79)μm]相比均明显减少(均 P<0.05),说明加入 NS398后细胞迁移能力下降。50μmol/L NS398组及100μmol/L NS398组与对照0μmol/L NS398组相比,COX-2、RhoA、CDC42、Rac-1表达均降低(均 P<0.05)。结论 NS398可抑制食管癌细胞 EC109迁移运动能力,其机制可能与抑制 COX-2表达,调节迁移基因 RhoA、CDC42及 Rac-1的表达有关。%Objective To investigate the effect of NS398,a COX-2 selective inhibitor,on the migration of EC109 cells(an e-sophageal carcinoma cell line)and the underlying mechanism.Methods EC109 cells were treated with NS398 at different con-centrations(0,50,100μmol/L).Cell wound-healing assay was used to observe the changes of cell migration within 48 h after treatment,and reverse transcription polymerase chain reaction(PCR)to detect the expression levels of COX-2,RhoA,CDC42 and Rac-1.Results The cell wound-healing assay showed that the migration distance was much less in 50μmol/L[24 h:(50.81 ±8.51)μm,48 h:(109.67±18.38)μm]and 100μmol/L[24 h:(53.15±9.58)μm,48 h:(97.42±13.20)μm]NS398 groups than in 0μmol/L NS398 group[24 h:(68.72±5.68)μm,48 h:(129.68±10.79)μm](P<0.05),suggesting the decline of the migrating ability of EC109 cells treated with NS398.The expression levels of COX-2,RhoA,CDC42 and Rac-1 were significantly decreased in 50μmol/L and 100μmol/L NS398 groups when compared with 0μmol/L NS398 group(P<0.05).Conclusion NS398 inhibits the migration of EC109 esophageal carcinoma cells by inhibiting the COX-2 expression and regulating the expression of migration-related genes RhoA,CDC42 and Rac-1.
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