湖南地区HBV患者MHC-Ⅰ类链相关基因A第五外显子微卫星多态性研究

摘要

Peripheral blood genome DNA were extracted form 108 chronic HBV infectious patients,96 HBV carriers and 142 healthy controls from Hunan Han population respectively, and the microsatellite polymorphism of exon 5 to major histocompatibility complex class I chain related A(MICA) were analyzed using polymerase chain reaction(PCR) and genes canning technique. The genotypes were confirmed by DNA sequencing, MICA gene deletion was detected by PCR-sequence specific priming (PCR/SSP) and the genotypes of MICA exon 5 were determined according to the three detecting results. The result showed that five alleles of MICA-STR were detected in this study, they are A4, A5, A5. 1, 46and A9, and the A5 and A5. 1 genes were the main allele gene. One MICA * Del gene was detected in both HBV carrier group and healthy control group. Genotypes frequency of MICA * A5. /A9 and allele frequency of MICA * A9 in chronic HBV patient group, phenotype frequency of MICA * A9 and gene phenotypic frequency of MICA * A9 in the HBV carrier group were significantly lower than that of in the healthy control group. While the genotypic frequency, allele frequency and phenotypic frequency of MICA exon 5 showed no significant difference between the HBV carrier group and the chronic HBV patient group. The results of this studies implied that MICA * A5. 1/A9 and MICA * A9 seem to be one of protective genotypes against HBV infection, this is the first discovery in the investigations of MICA exon 5, which provided a basis for further investigation of the infections, prevent and treatment.%分别提取湖南汉族人群108例慢性乙肝病毒(hepatitis B virus,HBV)患者、96例HBV携带者和142例健康对照者外周血基因组DNA,利用聚合酶链反应和基因扫描技术分别对它们的主要组织相容性复合体Ⅰ类链相关A(major histocompatibility complex class Ⅰ chain related A,MICA)基因第5外显子进行微卫星多态性分析；应用DNA测序分析对不同的基因型进行验证,同时应用PCR/SSP技术进行MICA*Del检测,确定MICA基因第5外显子基因型.发现本研究的三组中分别检出A4、A5、A5.1、A6、A9五种等位基因,且以A5和A5.1为主；在HBV携带组和健康对照组中分别检出MICA* Del基因.结果同时显示慢性HBV患者组MICA*A5.1/A9基因型频率、慢性HBV患者组的MICA*A9等位基因频率、慢性HBV患者组MICA* A9表型频率和HBV携带组MICA*A5.1/A9基因型频率均低于相应的健康对照组；而湖南地区汉族人群HBV携带者和慢性HBV患者间的基因型频率、等位基因频率和表型频率无显著性差异；因而推测MICA*A5.1/A9基因型和MICA*A9等位基因可能是抗HBV感染的一种保护性等位基因.为今后进一步研究HBV的感染、预防和治疗提供参考依据.