运用均匀设计法,对影响Rossetta(DE3)/pGEX-KG/AtGID1a表达水平的因素进行了优化,摸索出了对可溶性蛋白进行诱导表达的最优条件,即蛋白胨16 g/L,酵母粉5 g/L,葡萄糖4g/L,IPTG终浓度0.1 mmol/L,诱导温度23 ℃,IPTG诱导时间4 h.在此条件下,目的蛋白的平均密度从0.042提高到0.064,优化率为52%.%The factors that influenced expression level of Rossetta( DE3 )/pGEX-KG/AtGIDla were optimized using uniform design. The optimization induction condition for the solubility protein expression were investigated. The results were as followed: 16 g/L peptone, 5 g/L yeast powder, 4 g/L glucose, 0. 1 mmol/L IPTG, 23 ℃ induction tempera ture and 4 h induction time. The average density of interest protein had increased from 0.042 to 0. 064. The optimization rate was 52 ％.
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