Objective The polymorphism of microsatellite loci among four inbred strains of rats ( HFJ, MU, Lewis, F344) was analyzed using 24 pair primers. Methods To extract the genomic DNA of MU, HFJ, Lewis and F344 inbred rats by standard extraction procedure with phenol/chloroform. 24 microsatellite loci were selected for PCR amplification. Through native polyacrylamide gel eleclrophoresis and silver staining, microsatellite polymorphism was analyzed in the four strains of inbred rats. Results The amplified products of 24 microsatellite loci among different individual rats of these 4 strains and the same strain rats displayed single allelic gene band. Among the 24 microsatellite loci, it displayed mono-morphism between HFJ and MU rats. But HFJ and MU rats had significant deviations from Lewis and F344 rats. 14 micro-satellite loci turned out to be polymorphic, and 10 microsatellite loci displayed monomorphism. Conclusions The results of this study suggest that MU and HFJ strains basically meet the requirement of inbred strain. The 14 screened loci can be used for genetic monitoring of these inbred rats.%目的 用24对引物对近交系HFJ和MIJ大鼠的微卫星位点进行多态性分析,并选用近交系Lewis和F344大鼠作为对照,进行比较分析.方法 用传统的酚-氯仿法分别提取4个近交系大鼠MIJ、HFJ、Lewis和F344 的基因组DNA,选取大鼠24个微卫星位点,通过PCR扩增,扩增产物经过非变性聚丙烯酰胺凝胶电泳和银染,根据电泳结果,比较分析4种品系近交系大鼠之间微卫星多态性.结果 4种品系及品系内不同个体的近交系大鼠在24个微卫星位点上的扩增产物均出现一个条带,MIJ和HFJ大鼠在品系间和品系内均表现为单态性,同Lewis 和F344的扩增结果比较,14个位点显示多态性,有10个位点显示单态性.结论 两个近交系大鼠品系MIJ和HFJ符合近交系要求,筛选出的14个多态性微卫星位点可用于有关近交系大鼠的遗传背景监测.
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