大鼠局灶性脑缺血模型评价方法间关联性分析

摘要

Objective To analyze the correlation between three evaluation methods of middle cerebral artery occlu -sion and focal cerebral ischemia , and to explore a new method and standard for the evaluation of the model .Method Thirty healthy adult male SD rats were randomly divided into a sham-operated group ( n=20 ) and model group .According to Zea-Longa procedure ,we established the disease model to detect the changes in cerebral blood flow before and after sur -gery.The resulted cerebral infarction area was taken as gold standards .Then we analyzed the correlation between the standards and the changes in cerebral blood flow .Results The changes of infarction area ratio were positively correlated with the changes of cerebral blood flow in the model rats .Conclusions The changes of rat cerebral blood flow can be used to evaluate whether the cerebral ischemia model is successful or not .%目的 分析评价线栓法制备大鼠大脑中动脉阻塞模型成功的三种方法之间的相关性,为评价该模型提供一种新的方法和标准. 方法 雄性SD大鼠共(30)只,随机分成假手术组(n =15)、模型组(n=15) ,参照Zea-Longa线栓法稍加改进制备局灶性脑缺血模型,于术前术后检测各组大鼠脑血流变化. 以大鼠脑梗死面积率为金标准,分析该标准与脑血流变化之间的相关性. 结果 模型大鼠脑梗死面积率与脑血流变化量呈正相关,与行为学评分高低无关. 结论 大鼠脑血流量变化可作为评价局灶性脑缺血模型成功的标准.