目的：通过实验小鼠肾匀浆中苹果酸酶-1和异柠檬酸脱氢酶-1检测能力验证计划，了解实验动物检测机构检验能力，提高实验动物质量检测水平。方法按照CNAS批准的能力验证方案，通过样品制备，经过稳定性和均匀性检验合格，作为能力验证样品。采用随机编号，发样给参加单位，并附作业指导书。在规定时限提交检验报告和原始记录复印件，其结果与样品预检结果一致的判为满意结果，不一致或未能提交结果的判为不满意结果。结果共有10个实验室参加本次能力验证项目，其中满意结果的实验室8个，占总参加机构的80％，不满意的实验室有2个，占20％。结论实验动物质量检测机构在实验小鼠肾匀浆中苹果酸酶-1和异柠檬酸脱氢酶-1的检测能力较高，实施能力验证计划能够反映实验室的检测水平。%Objective To investigate the detection capacity of malic enzyme 1 and isocitrate dehydrogenase 1 ( Mod1&Idh1) in the laboratory animal monitoring laboratories in China in order to understand the detection capacity of la-boratories and to improve the detection level of laboratory animals’ quality.Methods Based on the program approved by CNAS, samples preparing, homogeneity test and stability test of malic enzyme 1 and isocitrate dehydrogenase 1 in the mouse kidneys were carried out.Standard operation procedure and samples with random numbers were distributed to the la-boratories.The laboratories should submit the result reports before the time limit expires.If the laboratory reports were the same with the standard results, the laboratories will receive satisfactory remark.If laboratory reports were not the same with the standard results, the laboratories will receive unsatisfactory remark.If a laboratory did not submit report, the laboratory will also receive unsatisfactory result.Results Eight laboratories out of 10 (80%) enrolled laboratories reported satisfac-tory experiment results, and two laboratories (20%) presented unsatisfactory results.Conclusions The whole detection level of laboratories in Mod1 &Idh1 is relatively high in the laboratory animals monitoring laboratories in China.It can re-flect the detection level of laboratories to conduct the laboratory capacity evaluation.
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