嗜热四膜虫接合生殖后期皮层细胞骨架蛋白的研究

摘要

Colchicine and cytochalasin B were used to investigate their influence on the conjugation of Tetrahymena thermophila, especially at the late stage. In the experimental group that was treated with colchicine, the percent of 34KD、37KD、46KD and 57KD cortical cytoskeletal proteins changed a lot, while in the experimental group treated with cytochalasin B, the percent of 40KD and 74KD proteins changed numeraely. According to some relevant papers, we inferred that 34KD、37KD、46KD and 57KD may be tubulin, while 40KD and 74KD may be microfilamental proteins, furthermore, these proteins are all important to the morphogenesis of Tetrahymena thermophila during conjugation, and it needs further investigation.%用秋水仙素和细胞松弛素B处理接合期的嗜热四膜虫,以观察其对接合生殖期,尤其是接合后期的嗜热四膜虫皮层细胞骨架蛋白的影响.用秋水仙素处理的试验组的皮层细胞骨架蛋白组分中34KD、37KD、46KD和57KD蛋白的含量有明显改变,而用细胞松弛素B处理的试验组中40KD和74KD蛋白的含量改变较大.根据相关文献,作者推测34KD、37KD、46KD、57KD蛋白是微管蛋白,而40KD和74KD可能是微丝蛋白.这些蛋白对嗜热四膜虫接合过程中的形态发生的重要作用有待进一步研究.