异育银鲫幼鱼对饲料中维生素B6需求量的研究

摘要

通过8周的生长实验,研究饲料中维生素B6的含量对异育银鲫(Carassius auratas gibelio)(初始体重3.3 g)生长、饲料利用及生化指标的影响.配制7组等氮等能的饲料,饲料中维生素B6的含量分别为0、1.11、3.29、5.41、12.7、13.3和27.3 mg/kg.实验结果表明,饲料中缺乏维生素B6会显著降低异育银鲫的特定生长率、饲料转化效率、蛋白质效率、存活率、肝体指数,但对摄食率没有显著影响.饲料中维生素B6的含量为12.70 mg/kg时,肝脏谷草转氨酶活性(GOT)活性最强,并且显著高于对照组(P＜0.05).在饲料中维生素B6含量为5.41 mg/kg时,肝脏谷丙转氨酶活性(GPT)活性显著提高.当饲料中维生素B6含量为12.7 mg/kg时,肝脏中维生素B6的沉积最高.通过折线法,根据特定生长率和饲料中维生素B6含量的关系,得出异育银鲫最大生长时饲料维生素B6的适宜含量为0.95 mg/kg;而以肝脏GOT和GPT活性为指标时,其饲料维生素B6的适宜含量分别为11.36和7.62 mg/kg.%An 8-week growth trial was conducted to determine the dietary VB6 requirement for gibel carp(Carassius auratas gibelio)(initial body weight of 3.3g).Seven iso-nitrogenous and iso-caloric experimental diets were formulated to contain different levels of VB6 (0, 1.11, 3.29, 5.41, 12.7, 13.3 and 27.3 mg/kg, respectively).The results showed that fish fed the control diet without supplementation of VB6 showed significantly lower specific growth rate, feed conversion efficiency, protein efficiency ratio, survival and hepatosomatic index than fish fed diets supplemented with VB6.The feeding rate exhibited no significant differences among the treatments (P ＞ 0.05).Fish fed the diet containing 12.70 mg/kg VB6 obtained significantly higher liver GOT activity than the other groups.The liver GPT activity significantly increased in fish fed diets containing ≥5.41 mg VB6/kg.Liver VB6 concentration was highest when fish fed the diet containing 12.7 mg/kg VB6.Based on the broken-line method of the relationships between specific growth rate, GOT and GPT activities and dietary VB6 concentrations, it can be concluded that the dietary VB6 requirement for gibel carp was considered to be 0.95, 11.36 or 7.62 mg/kg diet.