腐食酪螨螨体正己烷提取物的生物活性及成分分析

摘要

[目的]确定腐食酪螨Tyrophagus putrescentiae报警信息素的存在及其成分.[方法]采取正己烷溶剂浸提法提取获得腐食酪螨螨体提取物,通过观察腐食酪螨对提取物的行为反应确定其生物活性,最后用气相色谱-质谱联用仪(gas chromatograph-mass spectrum,GC-MS)对提取物的成分进行分析.[结果]腐食酪螨正己烷提取物对该螨有明显的报警作用.GC-MS分析显示,提取物中含有橙花醇甲酸酯、Z-柠檬醛、E-柠檬醛等成分.[结论]该实验证明了腐食酪螨报警信息素的存在,为进一步明确各成分及其作用奠定了基础.%[Aim] To explore the components of the alarm pheromone of Tyrophagus putrescentiae. [Methods] The extracts were extracted from T. putrescentiae mites with hexane. Biological activity of the extracts was assayed by observing the behavioral response of the mite to the extracts. The components of the extracts were identified by GC-MS. [Results] T. putrescentiae had intensive alarm response to the extracts, which consist of neryl formate, Z-citral, and E-citral based on GC-MS data. [Conclusion] This experiment proved the existence of alarm pheromone in T. putrescentiae and established the foundation for its further research.