桃蛀螟成虫Orco嗅觉受体基因的克隆及组织表达谱分析

摘要

[目的]克隆桃蛀螟Conogethes punctiferalis (Guenée)的Orco嗅觉受体基因,并研究其在不同组织的表达谱.[方法]利用PCR技术克隆桃蛀螟触角Orco基因,对该基因编码的氨基酸序列进行生物信息学分析,并利用荧光定量PCR技术分析该基因在的表达量.[结果]获得桃蛀螟成虫Orco的cDNA全长序列,并命名为CpunOrco(GenBank登录号:JX101681).该基因的开放阅读框全长1 425 bp,编码475个氨基酸,序列中有7个跨膜区.对桃蛀螟成虫不同组织中CpunOrco的荧光定量PCR结果表明,CpunOrco主要在触角和下颚须中表达,雄虫触角中的表达量高于雌虫,并且该基因在其他组织中也有一定的表达.[结论]本研究明确了该嗅觉受体基因在桃蛀螟成虫不同组织内的表达水平,为进一步研究其功能提供了理论依据.%[Aim]To clone the olfactory receptor co-receptor (Orco) gene in the yellow peach moth Congethes punctiferalis (Guenee) and analyze its expression level in different tissues. [Methods]The Oreo gene was cloned by polymerase chain reaction (PCR) , and its expression level was analyzed by real-time quantitative PCR. [Results]The Orco gene was cloned from adults of C. punctiferalis, and named as CpunOrco ( GenBank accession no. JX101681). Sequence analysis revealed that the open reading frame of CpunOrco is 1 425 bp in length, encoding 475 amino acid residues, with seven transmembrane domains. Real-time quantitative PCR results indicated that CpunOrco was mainly expressed in the antennae and maxillary palpi of C. punctiferalis, while expressed in other tissues at a relatively low level. The expression level of CpunOrco was higher in antennae of male adults than in antennae of female adults. [Conclusion ]This study makes clear the expression level of CpunOrco in different tissues of C. punctiferalis adults and provides a basis for further functional study of this gene.