五味子糖浆中五味子木脂素类成分的含量测定

摘要

目的：建立高效液相色谱法测定五味子糖浆中五味子醇甲、五味子醇乙、五味子甲素含量的方法。方法：采用月旭XDB C18（5μm，4．6mm ×250mm）色谱柱，流动相甲醇－水梯度洗脱，流速：1．0mL／min，检测波长：250nm。结果：五味子醇甲在0．04～2．02mg／mL范围内呈良好的线性关系（r＝1），回归方程为：y ＝10311 x－23．19。其平均回收率为98．37％（ RSD ＝1．519％）。五味子醇乙在0．011～1．76 mg／mL范围内呈良好的线性关系（ r＝0．9998），回归方程为：y＝10704 x－24．666。其平均回收率为101．625％（ RSD＝1．307％）。五味子甲素在0．004～0．81 mg／mL范围内呈良好的线性关系（ r＝1），回归方程为：y＝10601x－4．9829。其平均回收率为102．311％（ RSD＝0．824％）。结论：本方法简便、准确，可用于五味子糖浆中五味子木脂素类的含量测定。%Objective:To establish the determination method of Schizandrin, Aomisin and Deoxyschizandrin in Wuweizi syrup with RP-HPLC.Methods:The samples were analyzed on a XDB C18 column (5μm,4.6mm ×250mm) by using formaldehyde-water as the mobile phase at the flow rate of 1.0mL/min,250nm as the detection wave-length.Re-sults:Schizandrin showed a good relationship at the range of 0.04 ~2.02mg/ml (r=1).Regression Equation:y=10311x-23.19.The average recovery of syringin was 98.37%and RSD=1.519%.Aomisin showed a good relationship at the range of 0.011~1.76 mg/ml (r=0.9998).Regression Equation:y=10704x-24.666.The average recovery of syringin was 101.625%and RSD=1.307%.Deoxyschizandrin showed a good relationship at the range of 0.004~0.81mg/ml (r=1).Regression Equation:y=10601x-4.9829.The average recovery of syringin was 102.311% and RSD=0.824%.Conclusion:The method is simple,convenient and accurate,and can be used for content determination of Mixed ZiMu lipid classes in Wuweizi syrup.