目的：探讨齐墩果酸及环磷酰胺对H-22荷瘤小鼠的抗肿瘤作用机制。方法：肝癌H-22肿瘤细胞的小鼠随机分为模型组、环磷酰胺组、齐墩果酸组、齐墩果酸组与环磷酰胺联合应用组。连续给药8天后剥离瘤组织，采用免疫组化法检测肿瘤组织中突变型P53蛋白及肿瘤坏死因子（ TNF-α）的含量。结果：齐墩果酸组与环磷酰胺联合应用对H-22荷瘤小鼠TNF-α含量明显提高，与模型组比较差异显著（ P＜0．05）；齐墩果酸组与环磷酰胺联合应用对H-22荷瘤小鼠突变型P53蛋白表达明显降低，与模型组比较差异显著（P＜0．05）。结论：齐墩果酸组与环磷酰胺联合应用能下调H-22荷瘤小鼠肿瘤细胞内突变型P53蛋白的表达，对H-22荷瘤小鼠肿瘤细胞内TNF-α有明显的诱生作用。%Objective:To investigate the antitumor mechanism of oleanolic acid and cyclophosphamide on H -22 tumor bearing mice .Methods:H-22 tumor cells were randomly divided into model group , cyclophosphamide group , oleanolic acid group , combined group of oleanolic acid and cyclophosphamide .Continuous administration for eight days after the tumor tissue , immunohistochemistry of mutant P 53 protein and tumor necrosis factor in the tumor tissue were detected ( TNF-α) content .Results:By combined application of oleanolic acid and cyclophosphamide in mice H -22 TNF-αcontent increased significantly , and compared with the model group it had a significant difference (P<0.05);by com-bined application of oleanolic acid and cyclophosphamide the expression of H -22 mutant P53 protein was significantly decreased in mice, with marked difference compared with the model group (P<0.05).Conclusion:The expression of combined application of oleanolic acid and cyclophosphamide can down -regulate H-22 in tumor cells of mutant P 53 protein,which has an effect on the H -22 induced tumor cell of tumor bearing mice in TNF -α.
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