以马来松香丙烯酸乙二醇酯为交联剂的茶碱分子印迹膜电化学传感器的研究

摘要

Abstract A novel electrochemical sensor for the determination of theophylline based on ethylene glycol maleic rosinate acrylate as the cross linking agent and acrylic acid as functional monomer was fabricated by molecularly imprinted technology. A molecularly imprinted polymers （MIPs） membrane was synthesized on the surface of a glassy carbon electrode in vacuum drying oven by free radical polymerization method. The electrochemical behavior of the membrane was characterized by cyclic voltammetry （CV）, electrochemical impedance spectroscopy （EIS） and chronoamperometry. Under optimum conditions, it was found that the response of peak currents was linear to the concentration of theophylline in range of 2.00× 10 7-3.45 ×10-4 mol/L （linear regression coefficient= 0.9961） with the detection limit of 1.00 ×10-7 mol/L. The sensor not only has high selectivity, but also shows good stability and reproducibility. The sensor was applied to the determination of theophylline in theophylline sustained release tablets samples with recovery ranging from 95.6% to 103.8%.%利用分子印迹技术，以马来松香丙烯酸乙二醇酯为交联剂，丙烯酸为功能单体研制了测定茶碱的新型电化学传感器．以自由基热聚合的方式于玻碳电极表面制备茶碱分子印迹聚合物膜．通过循环伏安法、电化学交流阻抗法及计时电流法研究了传感器对茶碱的响应特性．结果表明，在最佳的实验条件下，传感器的峰电流与茶碱浓度在2．00×10^-7～3．45×10^-4mol／L范围内呈现出良好的线性关系（线性相关系数为0．9961），检出限（S/N=3）为1．00×10^-7mol／L；该传感器的选择性高，稳定性和重现性好，将此传感器用于茶碱缓释片中茶碱的含量测定，回收率为95．6％～103．8％．