Via the coupling effect of γ-aminopropyltrimethoxysilane,methacrylic acid was graft-polymerized onto the surface of silica gel particles,obtaining the grafted particles PMAA/SiO2.Then,the molecular imprinting towards the grafted PMAA was performed with one enantiomer of tetramisole（TM）,L-TM,as template molecule and ethylene glycol diglycidyl ether（EGDE） as crosslinking agent by using the novel surface-molecular imprinting technique established by our research group,and the L-TM molecule-imprinted material MIP-PMAA/SiO2 was prepared.With another enantiomer,D-TM,as a contrast compound,both static and dynamic methods were adopted to study the chiral recognition character and binding property of MIP-PMAA/SiO2 for L-TM and to examine its ability to separate the two enantiomers in racemate.The experiment results show that MIP-PMAA/SiO2 have specific recognition selectivity and excellent binding affinity for L-TM,and its selectivity coefficient for L-TM with respect to D-TM reaches 4.03,displaying fine ability to separate the two enantiomers.Besides,MIP-PMAA/SiO2 has excellent elution property,and with a diluted aqueous solution of NaOH as the eluent,the desorption ratio of L-TM reaches 99.08% in 20 BV.%通过偶联剂γ-氨丙基三甲氧基硅烷（AMPS）的媒介,通过表面引发接枝,将功能单体甲基丙烯酸（MAA）接枝聚合在微米级硅胶微粒表面,制得功能接枝微粒PMAA/SiO2;采用本课题组建立的新型分子表面印迹技术,以四咪唑（TM）为模型手性药物,用其左旋对映体L-TM为模板分子,乙二醇二缩水甘油醚（EGDE）为交联剂,对接枝在硅胶表面的PMAA大分子链进行了分子印迹,制备了对映体L-TM分子表面印迹材料MIP-PMAA/SiO2.探索了该印迹材料对外消旋体D,L-TM中两种对映体的拆分性能,采用静态与动态两种方法考察了MIP-PMAA/SiO2对模板分子L-TM的分子识别特性与结合性能.实验结果表明,印迹材料MIP-PMAA/SiO2对L-TM分子具有特异的分子识别选择性与优良的结合亲和性,相对于右旋对映体D-TM,识别选择性系数为4.03,MIP-PMAA/SiO2对外消旋体D,L-TM中的两种对映体表现出优良的拆分性能.此外,印迹材料MIP-PMAA/SiO2也具有良好的解吸性能,以稀NaOH水溶液为洗脱液,20个床体积内解吸率即达到99.08%.
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