以添加了不同浓度的甘露醇、PP333和ABA的培养基对半夏试管苗进行缓慢生长法保存,并对保存材料再生后代的体细胞变异进行检测.结果显示,甘露醇、PP333和ABA均能有效抑制试管苗生长,且存活率高;最佳浓度分别为甘露醇2％～4％,PP333 2.0 mg·L-1,ABA 2.0～4.0 mg·L-1.保存在添加了2％～4％甘露醇或2.0 mg·L-1 PP333培养基上的植株未检测到变异,而保存在添加了2.0～4.0 mg·L-1 ABA培养基上的植株检测到1条新增标记和1条缺失标记,位点变异率为1.7％,个体变异率为30％.研究表明,ABA不宜用于半夏试管苗的缓慢生长法保存,但有助于新突变体的产生,在种质创新上具有特殊意义.%Slow growth conservation of Pinellia ternata on medium by adding different concentrations of mannitol,PP333 , and ABA were carried out. Somatic variation of regenerated plantlets was also studied. The results show that:the three of them all could effectively retard the growth with a high surviving rate. The optimum concentration of mannitol,PP333 and ABA were 2%～4% ,2. 0 mg ·L-1 and 2. 0～4. 0 mg ·L-1, respectively. No variations were found in the regenerated plantlets which were conserved on medium by adding mannitol 2% or PP333 2. 0 mg ·L-1. While,one new marker and one absent marker were found on medium by adding ABA 2. 0 mg ·L-1. The locus variation rate was 1. 7% and the individual variation rate was 30%. Therefore,ABA was not suggested in slow growth conservation of Pinellia. However,it contributed to the generation of new mutant, and has a special significance in the innovation of P. ternate germ-plasm.
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