唐菖蒲赤霉素受体基因克隆及表达分析

摘要

以唐菖蒲子球为材料,采用 RT-PCR 技术,克隆了1个赤霉素(GA)受体基因,命名为 GhGID 1a (GenBank登录号为 KU525107)。其开放阅读框为1032 bp,编码343个氨基酸。序列比对结果表明,GhGID1a 推导氨基酸序列与百子莲、油棕和海枣等植物的 GID1的氨基酸序列相似性较高,分别为82%、78%和77%。50 mg/L GA3对子球萌发具有促进作用,150 mg/L GA3会抑制其萌发。实时荧光定量 PCR 结果显示,GA3处理对 GhGID 1a 基因表达具有反馈抑制作用,GhGID 1a 表达量随着子球休眠解除而逐渐降低,推测唐菖蒲子球休眠解除可能与 GA 及其受体基因有关,GA 及其受体基因 GhGID 1a 可能参与了调控唐菖蒲的子球休眠与萌发过程。%Using RT-PCR technique,we cloned a gibberellin receptor gene from cormels of Gladiolus hybri-dus named as GhGID 1a (genbank accession number KU525 107).The open reading frame of GhGID 1a was 1 032 bp,which encodes 343 amino acids.The sequence alignment results indicated that the deduced amino acid sequence of GhGID1a was similar to the GID amino acid sequences of Agapanthus praecox ,Elaeis guineensis and Phoenix dactylifera ,with the similarity of 82%,78% and 77%,respectively.50 mg/L GA3 could promote cormel germination,but GA3 at 150 mg/L could inhibit germination.Real-time PCR results indicated that GA3 treatments caused feedback-inhibition on GhGID 1a expression.The GhGID 1a expression gradually decreased with cormel dormancy release,which inferred that GA and its receptor gene GhGID 1a participated in the regulation process of Gladiolus hybridus cormel dormancy and germination.