果桑肥大性菌核病菌和油菜菌核病菌的交叉侵染、生物学特性及遗传关系

摘要

The cross infection was studied between ascospores ofCiboria shiraiana causing hypertrophy sorosis sclerotenisis in mulberry andSclerotinia sclerotiorum causingSclerotinia stem rot in oilseed rap. Inoculation results showed that ascospores ofC. shiraiana infected oilseed rape, and ascospores ofS. sclerotiorum infected sorosis similarly. Conidia and conidiophores appeared in the infected sorosis byC. shiraianaand S. sclerotiorum, but did not appear in the infected oilseed rape. The cross section showed a structure of capsule ascus round cell inC. shiraianaunder microscope, but the texture angularity inS. sclerotiorm. Clustering based on SRAP of isolates from southwest areas showedC. shiraiana isolates from sorosis was classified into one group, andS. sclerotiorm isoplates from oilseed rape into a second group, with an except where oneC. shiraiana isolate and oneS. sclerotiorm isolate were clustered into an additional group. Cross infection of the two host plants by these two pathogen ascospores suggests that intercropping or interplanting of mulberry and oilseed rape is not feasible where the two pathogens/diseases exist.%分别以果桑肥大性菌核病菌和油菜菌核病菌的子囊孢子交叉接种油菜和果桑。结果表明，杯盘菌子囊孢子能够侵染油菜，同样，核盘菌子囊孢子能够侵染果桑；在受侵染的桑椹上2种病菌均产生分生孢子梗和分生孢子，而在油菜上不产生。显微镜观察，杯盘菌的子囊盘外囊被切面为圆胞组织结构，核盘菌为角胞组织结构。SRAP分子标记和聚类结果表明，采自西南地区果桑上的杯盘菌和油菜上核盘菌基本各自聚在一类，其中一个杯盘菌分离物和一个核盘菌分离物聚在一类，表现特别。2种寄主上的病菌子囊孢子能相互侵染，因而果桑和油菜不能间套种植。