水稻温敏黄转绿突变体v5的鉴定和基因定位

摘要

A thermo-sensitive vireseent rice mutant was identified from M2 of Nipponbare by 60Crγ radiation, temporarily named as v5(virescent 5). The leaf color of the mutant is yellow at lower temperature (20 -24t) while turning to nearly normal green at higher temperature (26 - 30t). The chlorophyll content of the mutants was significantly lower than wild-type plants under lower temperature. The agronomic traits showed that the yield of individual mutant was significantly lower than wild-type. It was revealed from the genetic analysis that it was a single nuclear-encoded recessive gene. It's responsible for the mutation and mapped on chromosome 9. A high-resolution physical map of the chromosomal region around the OsV5 gene was then constructed using F2 population (v5/Kasalath ) and the gene was finally located on the region of 166. 5 kb between the marker STS1 on the BAC AP005838 and the marker STS5312 on the BAC AP005702.%通过对梗稻品种‘日本晴’进行60Coγ射线诱变,从M2中筛选到一个温度敏感的黄转绿突变体v5(virescent5).突变体植株在较低温度(20～24℃)下叶色表现为黄色,而植株在较高温度(26～30℃)下叶色几乎表现为绿色.对叶绿素含量的测定显示出低温条件下突变体叶绿素含量显著低于野生型植株；对相关农艺性状考察显示出突变体单株产量显著低于野生型.遗传分析表明,该突变表型受一对隐性核基因控制,利用F2群体(v5/Kasalath)将OsV5基因定位于第9染色体长臂,在BAC AP005838上标记STS1与BACAP005702上标记STS5312之间166.5 kb的区段上.