在猪输卵管上皮细胞(pOECs)与猪卵巢皮质细胞(pOCCs)共培养体系中进行猪卵子体外成熟(IVM),研究比较了pOECs与pOCCs对猪卵母细胞体外成熟(IVM)、体外受精与胚胎发育的影响.结果显示:pOCCs或pOECs参与猪卵子体外成熟可显著提高卵丘扩散与MⅡ期卵子的比例(P＜0.05),然而GV期卵子的比例却没有显著减少(P＞0.05);pOCCs组内成熟卵子的GSH含量显著高于其余各组(P＜0.05),而GSH在pOECs组与对照组之间差异不显著(P＞0.05);尽管pOCCs和pOECs共成熟可显著降低多精入卵的比例(P＜O.05),但是受精率与雄原核形成率(MPN)在各组间却无显著差异(P＞0.05);pOCCs和pOECs共培养均可以显著提高卵裂率与囊胚发育率(P＞0.05),然而囊胚细胞数在处理组与对照组间没有显著性差异(P＞0.05).研究认为,pOCCs与pOECs共培养体系能显著提高猪卵母细胞IVM质量,降低多精入卵的发生,并提高囊胚发育率.%Porcine oocytes were co-cultured respectively with porcine ovarian cortex cells (pOCCs) and porcine oviductal epithelial cells (pOECs) during in vitro maturation (IVM).The effects of pOCCs and pOECs co-culture during IVM on the maturation,fertilization and embryos development in vitro were studied.The results showed that cumulus significantly expanded and matured and M Ⅱ oocytes in the co-culture groups were higher than that in the control group (P ＜ 0.05),whereas there was no significant difference in unmatured GV oocytes between the groups (P ＞ 0.05).In addition,the GSH concentration in the pOCCs group was higher than that in other groups (P ＜ 0.05).After IVF,polyspermy in the co-culture groups were lower than that in the control group (P ＜ 0.05),and there were no significant difference in both fertility and male pronucleus (MPN) formation between the groups (P ＞ 0.05).In conclusion,the presence of the pOCCs and pOECs during IVM enhanced the maturation quality of the porcine oocytes,reduced the polyspermy,and increased the percentages of blastocyst.
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