尿素包合法分离纯化山核桃油中亚油酸的研究

摘要

Both single factor and orthogonal experiments were used to optimize conditions for purification of linoleic acid from Carya cathayensis Sarg. oil by urea adduction fractionation. Effects of adduction temperature, time, the ratio of fatty acid and urea and the ratio of methanol and urea on the purity and yield of linoleic acid were investigated. The results showed that the optimal purification conditions were the ratio of fatty acid and urea (m/m) at 0. 3, the ratio of methanol and urea ( V/m) at 2. 5, adduction temperature at - 5℃ , and adduction time of 24 h, in which content of linoleic acid in Carya cathayensis Sarg. oil increased from 23. 80% to 80. 73% .%为优化尿素包合纯化山核桃油中亚油酸的工艺,采用单因素和正交试验,研究了尿素包合反应中温度、时间、脂肪酸/尿素、甲醇/尿素等因素对亚油酸纯度及得率的影响.结果表明:在m(脂肪酸)/m(尿素)为0.3,V(甲醇)/m(尿素)为2.5,包合温度为-5℃,包合时间为24h的优化条件下,山核桃油中亚油酸的含量由23.80％提高到80.73％.