为调查红原地区牦牛隐孢子虫感染情况,采用巢式PCR对采自红原县的216头份牦牛粪便进行了检测(其中包括腹泻粪便样本18份)。该地区牦牛隐孢子虫总感染率为14.8%,其中Cryptosporidium bovis感染率为3.7%,C. ryanae感染率为5.1%, C. andersoni感染率为6.0%,而腹泻样本并未检出隐孢子虫。以上结果表明,牦牛是隐孢子虫的天然宿主,但该实验中未见隐孢子虫和牦牛腹泻间存在明显相关性,隐孢子虫和牦牛腹泻之间的关系需要后续研究来进一步阐明。%In order to investigate prevalence of Cryptosporidium spp. in yaks in Hongyuan County, in this study, Cryptosporidium isolates from 216 fecal samples were identified by nest-PCR method ( including 18 diarrhea fecal samples) . Total infection rate of Cryptosporidium was 14. 8%. It showed that prevalence of Cryptosporidium bovis, C. ryanae and C. andersoni was 3. 7%, 5. 1% and 6. 0%, respectively. However, no Cryptosporidium spp. was de-tected in 18 diarrhea fecal samples. Above results showed that yaks were natural hosts of Cryptosporidium spp. . But in this study, no obvious correlation existed between Cryptosporidium spp. and diarrhea, and the relationship between Cryptosporidium spp. and diarrhea in yaks should be further explored.
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