利用DDRT-PCR技术初步分析了小麦条锈病抗性品种‘89-27'和耐性品种‘咸农四号’在感染后叶片中的基因差异表达,共筛选到差异显示片段47个,包括诱导表达8个、增强表达21个和抑制表达18个.其中,7个稳定表现的差异片段DD1～DD7测序和序列相似性比对结果表明:DD1与far1基因相似性较高,可能与Phy A蛋白调控的特异性生理活性有关；DD2与rRNA基因完全同源,可能与感染后应激蛋白大量快速合成有关；DD7与光合系统Ⅱ中的叶绿素结合蛋白a/b基因相似性较高,可能与光捕捉效率和CO2固定能力的补偿作用有关；DD3～DD6功能不详,可能为新EST序列.%The gene expressions of the leaves infected with P. striiformis were compared by the DDRT-PCR technology between the cultivar ' 89-27' resistant to stripe rust and the cultivar ' Xian-nong 4' tolerant to stripe rust,and it was found that there were 47 differential display fragments,including 8 induced expressions, 21 enhanced expressions and 18 inhibited expressions. The sequencing and sequence alignment of the 7 stable-expression fragments DD1 - DD7 showed that the DD1 was quite similar to the gene far1 and might be related to some specific physiological activities regulated by Phy A;The DD2 and the rRNA gene were completely homologous and might be related to large and fast synthesis of stress protein after infection;The DD7 was very similar to chlorophyll a/b-binding protein of Photosystem II and might be related to the compensation action of light-receiving efficiency and CO2 fixation capacity;The DD3 - DD6 were not clear in functions and might be new EST sequences.
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