一个水稻抗逆候选基因OsHMGB3的鉴定与评价

摘要

OsHMGB3是一个位于水稻抗旱QTL区段内的抗旱相关候选基因,预测编码一个具有HMGB-UBF-HMG-box结构域的蛋白.Real-time PCR分析表明:OsHMGB3基因在多个组织器官中表达,且能够受到PEG、NaCl、低温、高温等逆境及ABA的诱导表达.对超表达OsHMGB3的转基因水稻进行了苗期甘露醇胁迫处理,转基因植株的鲜重和株高均极显著优于野生型植株,超表达OsHMGB3可提高水稻苗期抵抗渗透胁迫的能力.该基因对逆境的响应及转基因植株对渗透胁迫的抗性均暗示该基因与水稻抗逆性有密切的关系.%OsHMGBS is located in a drought-resistant QTL segment of rice,putatively coding a domain protein with HMGB-UBF-HMG-box structure. The real-time PCR analysis showed that the expression of OsHMGB3 occurred in several plant organs and could be induced by PEG,NaCl,cold,heat and ABA. The mannitol stress treatment at seedling stage indicated that the O.s//MGB3-overexpression transgenic plants' plant height and fresh weight were very significantly superior to the wild-type plants'.Both the response of the gene to adverse environment and the resistance of the transgenic plants to osmotic stress implied that the gene is closely related to adverse resistance of rice.