高温胁迫对茄子果皮活性氧代谢、花青素及其主要合成酶的影响

摘要

Taking eggplant variety "Tewangda" as experimental material, the author studied the effects of high-temperature stress on the active oxygen metabolism, anthocyanin content and activity of its main synthases in eggplant peel. The results indica-ted that:high-temperature stress increased the malondialdehyde ( MDA) content, superoxide anion ( O2 ·-) production rate and hydrogen peroxide ( H2 O2 ) content in eggplant peel, enhanced the activities of superoxide dismutase ( SOD) , catalase ( CAT) , peroxidase ( POD) and ascorbate peroxidase ( APX) in eggplant peel, improved the contents of proline and soluble protein, re-duced the anthocyanin content and the activities of chalcone synthase ( CHS) , dihydroflavanonol 4-reductase ( DFR) , anthocya-nidin synthase ( ANS) and flavonoid 3-O-glucosyltransferase ( 3GT/UFGT) in eggplant peel, and strengthened the activity of phenylalanine ammonialyase ( PAL) .%以茄子品种'特旺达'为试材,研究了高温胁迫对茄子果皮活性氧(ROS)代谢、花青素含量及其主要合成酶活性的影响.结果表明:高温胁迫增加了茄子果皮丙二醛(MDA)含量、超氧阴离子(O2·-)产生速率和过氧化氢(H2 O2)含量,增强了茄子果皮中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)的活性,提高了脯氨酸和可溶性蛋白质含量,降低了果皮花青素含量以及查尔酮合成酶(CHS)、二氢黄酮醇还原酶(DFR)、花青素合成酶(ANS)和类黄酮3-葡糖基转移酶(3GT/UFGT)的活性,增强了苯丙氨酸解氨酶(PAL)的活性.