大白菜开花相关基因CO的BAC克隆筛选及分析

摘要

根据已经公布的大白菜CO基因(Bra008669)序列设计特异引物,采用三维PCR法筛选大白菜BAC文库,获得了3个含有CO的BAC克隆.对筛选到的3个BAC克隆进行PCR扩增,将克隆测序结果与大白菜CO基因进行序列比对,结果表明,相似性达到99.73％,且N端有保守B-BOX结构,C端有保守的CCT结构域,证明所筛选的3个BAC克隆是含有大白菜CO基因的克隆.%The specific primers designed according to the published Chinese cabbage CO gene ( Bra008669) were used to screen the Chinese cabbage BAC library through three steps of PCR, and 3 clones containing CO gene were obtained. The 3 obtained clones were amplificated and the sequence compared with Chinese cabbage CO gene (Bra008669) , the result showed that the amplificated fragments had 99. 73% of homology with the CO gene (Bra008669) and included N-terminal and C-terminal conservated domains.