断奶仔猪多系统衰竭综合征中多病原混合感染的流行病学调查

摘要

根据GenBank上发表的猪圆环病毒2型(Porcine circovirus-2,PCV2)基因组序列、猪博卡病毒(Porcine bocavirus,PBoV)的VP1、VP2基因序列和TTV1、TTV2的UTR序列设计合成引物,建立了分别用于检测PCV2、PBoV及TTV1、TTV2的PCR方法.应用建立的PCR方法对送检的205份猪血液和组织样品进行PCV2、PBoV、TTV1和TTV2的检测,并对临床病料和健康猪群进行了比较,综合调查了PMWS猪群中多病原混合感染的情况.试验结果表明,PCV2、PBoV、TTV1、TTV2的阳性率分别为:56.59％,38.05％,26.83％,34.63％.其中,较高的混合感染为:PCV2与PBoV(15.61％)以及PCV2与TTV2(16.59％).表明该地区PBoV和TTV2感染率升高,且混合感染种类复杂,从而为有效预防和控制这些疾病提供了理论依据.%According to the published genomic sequences of Porcine circovirus type 2(PCV2) ,the VP1 ,VP2 sequence of porcine bocavirus and the UTR of swine Torque teno virus ( TTV) , the primers were designed and a PCR were set up for the detection of PCV2 , PBoV and TTV, respectively. 205 samples collected from fields were detected for PCV2,PBoV and TTV by PCR. The multiple pathogens mixed infection were researched by the comparing the diseased and healthy pigs. The results demonstrated that the positive rate of PCV2,PBoV,TTV1 and TTV2 were 56. 59% ,38. 05% ,26. 83% and 34. 63% .respectively. The high co-infection rates were PCV2 + PBoV (15. 61% ) and PCV2 + TTV2 ( 16. 59% ). It indicated that PBoV and TTV2 infections were significantly increased in these regions and the co-infection of different viruses was complicated. It provided a theoretical basis for effectively controlling these diseases.