放线菌 Yn168菌种鉴定及其发酵条件对抗病毒活性的影响

摘要

通过对一株具有广谱抗病毒活性的放线菌菌株Yn168的形态、培养特征、生理生化特征及16 S rDNA序列分析，对该放线菌进行了菌种鉴定，并研究了不同发酵条件对该菌发酵液抗病毒活性的影响。鉴定结果表明，该菌株为一株小链霉菌（ Streptomyces parvus）。不同发酵条件下发酵液抗病毒活性检测表明，当种子培养24 h、种子液接种量为8％、培养温度为28℃、初始pH值为7．0、摇床转速为200 r/min时，发酵液对烟草花叶病毒（ Tobacco mosaic virus ， TMV）的抑制活性最强，枯斑抑制率在86％以上。%A actinomycete strain Yn168 with broad-spectrum antiviral activity was identified as Streptomyces parvus by culture characteristics , physiological and biochemical characteristics , and 16 S rDNA sequence analysis . Also ,the influence of different fermentation conditions on antiviral activity of the fermentation broth was studied .The experiment results indicated that the antiviral activities of the fermentation broth was optimal when the strain age was 24 h,inoculum was 8%,temperature was 28℃,initial pH was 7.0,rotary shaker was at 200 r/min.And the vi-rus inhibition rate was above 86%by tobacco mosaic virus ( TMV) necrosis test .