To study the susceptibility of ATG16L1 gene to non-specific digestive disorder ( NSDD) in rabbit, single nucleotide polymorphism ( SNP) of rabbit ATG16L1 gene was firstly detected by PCR-HRM and the associa-tion analysis of ATG16L1 gene mRNA expression in colon of fibre-deficient diet group was conducted .The results found that there was a SNP locus ( c.1482 G>A) in ATG16L1 gene and the SNP locus was associated with the sus-ceptibility to NSDD;the allele A of c.1482 G>A could increase the susceptibility (OR =1.43,95%confidence interval:1 .08-1 .91 , P <0 .05 );in recessive genetic model , the genotype AA could increase the susceptibility (OR =1.69,95%confidence interval:1.05-2.78,P <0.05);along with the increasing severity of non-specific digestive disorder,the ATG16L1 gene mRNA expression in colon was gradually increased (P <0.05);genotype AA had the lowest mRNA expression in fibre-deficient diet group ( P <0 .05 ) .These results showed that ATG16 L1 gene was associated with the genetic susceptibility to NSDD in rabbit .%为了研究家兔ATG16 L1基因对非特异性消化道紊乱的易感性，试验采用PCR-HRM 技术，首次对家兔ATG16L1基因进行单核苷酸多态性（ SNP）检测，同时结合低纤维群体结肠ATG16L1基因 mRNA表达量进行关联分析。结果表明：ATG16L1基因存在1个SNP位点（ c．1482 G＞A）与NSDD的易感性相关联；c．1482 G＞A位点A等位基因能增加NSDD的易感性（OR ＝1．43，95％置信区间：1．08～1．91，P ＜0．05）；在隐性遗传模型中，AA基因型能增加NSDD的易感性（OR ＝1．69，95％置信区间：1．05～2．78，P ＜0．05）；ATG16L1基因在结肠中的mRNA表达量随着炎症的加剧显著升高（P ＜0．05）；AA基因型在整个低纤维诱导NSDD组中的表达水平最低（P ＜0．05）。这些结果均表明，ATG16L1基因与家兔NSDD的遗传易感性相关。
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