PyTPS 转化花生对其后代耐盐性和品质性状的影响

摘要

In this experiment,to analyze the effects of PyTPS from Porphyra yezoensis on the salt tolerance and quality characteristics of seed of peanut ,the recombinant expression vector pCAMBIA 2300-PyTPS harboring PyTPS was transformed into peanut via pollen-tube pathway method .Transgenic plants of T 0 generation were analyzed by PCR and the positive rate was 34.7%.Transgenic seeds of T2 generation were tested for salt tolerance in a 0.7%NaCl solution and for quality by near infrared spectroscopy ,and the expression level of PyTPS gene in transgenic plants was analyzed by semi-quantity RT-PCR.These results suggested that the germination rate was substantially higher for the seeds from some transgenic plants than for seeds from the non-transgenic parent in the NaCl solution and transcription of PyTPS gene was detected in some transgenic plants .Significant changes were found in quality characteristics including protein content , oleic acid and linoleic acid content , oil content of some T 2-generation transgenic seeds .In a word,transgenic seeds of peanut exhibited enhanced tolerance to salinity stress and significant variation in quality characteristics after ectopic expression of the PyTPS gene in peanut .%为了研究条斑紫菜TPS基因（ PyTPS）对花生种子耐盐性和品质性状的影响，利用花粉管通道法，将携带条斑紫菜PyTPS基因的pCAMBIA2300-PyTPS重组载体导入花生，获得了T0转基因植株。利用PCR对T0植株进行了检测，PCR阳性率为34．7％。将部分转基因植株的T2种子进行耐盐试验，利用半定量RT-PCR分析了PyTPS基因在转基因植株中的表达情况，并用近红外技术测定了T2种子的品质性状。结果表明，部分转基因花生种子在盐溶液中的发芽率显著提高，PyTPS基因可在有些转基因植株中表达，有的转基因植株的蛋白质、油酸、亚油酸或脂肪含量发生了明显变化。总之，条斑紫菜PyTPS基因导入花生后，既可提高转基因花生种子的耐盐性，又对部分后代种子的品质性状产生影响。