绵羊 PRLH 及其受体基因的克隆及在下丘脑-垂体-性腺轴中差异表达的研究

摘要

To explore the expression of PRLH and its receptor gene in sheep gonadal axis , we successfully cloned the PRLH and receptor gene in organizations at the hypothalamic-pituitary-gonadal axis by used the sheep as experimental animal ,got the phylogenetic tree .Also we used qRT-PCR technology to research different expression of PRLH and its receptor gene in different tissues at HPG axis .The results showed that sheep PRLH and PRLHR nu-cleotide sequences of mRNA were 113,237 bp.PRLH gene nucleotide sequence and goats ,cattle,human,mouse ho-mology were 96.8%,93.3%,80.3%,74.6%.PRLHR gene and the Tibetan antelope , goat, cattle, human and mouse homology were 100%,99.3%,97.3%,90.4%,84.4%.PRLH and its receptor gene in sheep hypothala-mus,pituitary,uterine and ovary were expressed ,and the expression of PRLH gene in hypothalamus ,pituitary and o-vary was significantly less than the uterine ( P<0 .05 ) .Expression of PRLHR in uterus and pituitary were signifi-cantly different (P<0.05),no significant difference in other tissues (P>0.05).So the hypothalamus,pituitary,o-vary and uterus were the main organ for the synthesis of secretion .%为了探讨PRLH及其受体基因在绵羊性腺轴中的表达，以绵羊下丘脑-垂体-性腺轴系为研究对象，克隆了PRLH及其受体基因PRLHR，构建了系统进化树，并利用qRT-PCR技术对PRLH及其受体基因在绵羊性腺轴中不同组织的表达差异做了研究。结果表明，绵羊PRLH和PRLHR mRNA核苷酸序列分别为113，237 bp，PRLH基因核苷酸序列与山羊、牛、人、小鼠的同源性分别为96．8％，93．3％，80．3％，74．6％，PRLHR基因与藏羚羊、山羊、牛、人和小鼠的同源性分别为100％，99．3％，97．3％，90．4％，84．4％；PRLH及其受体基因在绵羊的下丘脑、垂体、子宫和卵巢中均有一定量的表达，且PRLH基因在下丘脑、垂体和卵巢中的表达要显著低于子宫（ P＜0．05）；PRLHR在子宫与垂体中的表达差异显著（ P＜0．05），其他差异不明显。说明下丘脑、垂体、子宫和卵巢是PRLH合成分泌和发挥作用的主要器官。