6-BA对葡萄果实中有机酸积累及相关基因表达的影响

摘要

In order to study the effect of 6-BA on grape fruit organic acid accumulation,and the possible mo-lecular mechanism of 6-BA regulating organic acid synthesis, a grape cultivar, Rizamat was selected to study the effect of 6-BA on fruit organic acid accumulation and related genes expression. The inflorescences and fruit clusters were treated with 6-BA at 5 days before blooming,3 and 10 days after anthesis respectively. The results showed that organic acid content of the mature grape fruit was closely related to treatment concentration of 6-BA. Tartaric acid and total organic acid content of the mature fruits that treated by 30 mg/L 6-BA was significantly lower than control. But it didn′t play important roles in the content of malic acid,citric acid and oxalic acid. For 10,20 mg/L 6-BA treatment,all kinds of organic acid didn′t show significant difference between treated and control mature fruits. Gene expression result showed that the expression of IDH,MDH and PEPC were mainly suppressed by 6-BA treatment,but it also promoted the expression of ME. Therefore,we speculated that the reason for inhibiting organic acid synthesis was that parts of the organic synthesis related genes expression were affected by 6-BA treatment.%为探究6-BA对葡萄果实中有机酸积累的影响,及其调控果实有机酸合成的分子机制,以里扎马特葡萄为试材,花前5 d、花后3 d和花后10 d连续3次对花序与果穗进行6-BA处理,研究6-BA对葡萄果实中有机酸积累及其代谢相关基因表达的影响.结果表明,成熟果实中有机酸含量与6-BA处理浓度密切相关,30 mg/L处理后,成熟果实中的酒石酸和总酸含量均显著低于对照,但对苹果酸、柠檬酸和草酸含量并未产生较大影响;对于10,20 mg/L 6-BA处理而言,处理与对照成熟果实中的各种有机酸含量均不存在显著差异.基因表达结果表明,6-BA处理对IDH、MDH和PEPC基因的表达总体表现为抑制,但对于ME基因的表达则主要表现为促进.据此分析,6-BA处理抑制果实中有机酸合成的原因可能与其影响部分有机酸代谢相关基因的表达有关.