大叶补血草Na~+/H~+逆向转运蛋白基因NHX1的克隆及番茄的遗传转化

摘要

根据NCBI公布的大叶补血草NHX1基因序列设计特异性引物,经RT-PCR方法克隆得到大叶补血草液泡膜Na~+/H~-逆向转运蛋白基因NHX1,构建植物融合表达载体pCAMBIA1301-NHX1,重组质粒通过农杆菌介导转化番茄,经PCR和RT-PCR鉴定,获得转基因番茄植株.本研究为大叶补血草耐盐基因NHX1的进一步功能分析和应用奠定了一定的理论基础.%Specific primers were designed according to gene NHX1 of Limonium gmelinii published on NCBI. Then, the gene was cloned by means of RT-PCR. Eukaryotic expression vector pCAM-BIA1301-NHX1 had been constructed, then transformed into tomato by Agrobacterium-mediated transformation method. Transgenic tomatos were obtained. It laid a theoretical foundation for further analysis and application of Limonium gmelinii NHX1 gene from this study.