桃RAPD扩增产物的不同电泳检测及其序列特征

摘要

In order to utilize RAPD technology better in identification of peach cultivars and genetic analysis, the RAPD fingerprints of 16 peach cultivars from agarose and polyacrylamide gel electrophoreses were compared, which was carried out with the newest optimization of RAPD technique in choosing 11 nt primers and strict screening PCR annealing temperature. The results showed that the polyacrylamide gel electrophoresis (PAGE) could detect PCR products better by presenting much more bands and more polymorphic bands could be uncovered. The random sequencing of 19 fragments showed that a mon g which six sequenceswere regions of encoding protein genes, suggesting RAPD can amplify both protein-encoding gene and non-protein encoding sequences, reflecting the ability of RAPD in uncovering the genetic difference of different peach cultivars with un-bias DNA information.%以16个桃品种为试验材料,选用11个碱基引物以及严格筛选PCR退火温度的RAPD技术,对琼脂糖凝胶及聚丙烯酰胺凝胶电泳检测的RAPD、PCR扩增产物进行比较.结果表明,聚丙烯酰胺凝胶电泳检测出的总谱带及多态性谱带数均高于琼脂糖凝胶,前者不存在或少存在共迁移性的现象.随机选取19个片段,发现其中有6条是编码蛋白的基因片段,说明RAPD不仅扩增基因组上的非编码蛋白序列,同时可扩增编码蛋白的基因片段,能够较全面客观地反映不同桃品种遗传上的差异.