为了分析抗秆锈病小种Ug99的基因在青海审定小麦和新合成的人工小麦中的分布状况,采用6个抗Ug99基因(Sr33、Sr36、Sr39、Sr40、Sr42、Sr43)的分子标记对青海省审定的66个小麦品种及86份人工合成小麦材料进行检测.结果表明:在青海审定的66个小麦品种中,有4个品种含Sr33,占检测品种数的6.06％;1个品种含Sr39,占检测品种数的1.52％;5个品种含Sr40,占检测品种数的7.58％;4个品种含Sr42,占检测品种数的6.06％;3个品种含Sr43,占检测品种数的4.55％;同时含有2个抗性基因的品种仅有2个,占检测品种数的3.03％.在86份合成小麦材料中,有38份材料含Sr33,占检测材料的44.19％;31份材料含Sr39,占检测材料的36.05％;9份材料含Sr40,占检测材料的10.47％;3份材料含Sr42,占检测材料的3.49％;6份材料含Sr43,占检测材料的6.98％;同时含有2种抗性基因的材料有17份,占检测材料的19.77％.在66个小麦品种和86份人工合成小麦材料均未检测出Sr36.人工合成小麦提供Ug99抗病基因资源,对改良普通小麦Ug99抗病性具有潜在价值.%To analyze the distribution of stem rust race Ug99 resistance genes in spring wheat cultivars which were released in Qinghai province and the new synthetic hexaploid wheat lines,six molecular markers of Ug99 resistant genes (Sr33,Sr36,Sr39,Sr40,Sr42,Sr43) were applied to test sixty-six wheat cultivars which were released in Qinghai province and eighty-six synthetic hexaploid wheat lines.Of the sixty-six Qinghai released cultivars,four cultivars carry Sr33,one cultivars carry Sr39,five cultivars carry Sr40,four cultivars carry Sr42,and three cultivars carry Sr43,accounting for 6.06％,1.52％,7.58％,6.06％,and 4.55％ of the detected samples,respectively.Only two cultivars contain two resistance genes,accounting for 3.03％ of the number of detected cultivars.In eighty-six synthetic hexaploid wheats,thirty-eight lines contain Sr33,accounting for 44.19％.Thirty-one lines contain Sr39,accounting for 36.05 ％.Nine lines contain Sr40,accounting for 10.47％.Three lines contain Sr42,accounting for 3.49 ％.Six lines contain Sr43,accounting for 6.98 ％.Meanwhile,seventeen of eighty-six synthetic hexaploid wheat lines contain two resistance genes,accounting for 19.77％.Sr36 was not detected in both sixty-six bread wheat cultivars and eighty-six synthetic hexaploid wheat lines.The results indicated that synthetic hexaploid wheat could be a potential germplasm for wheat Ug99 resistance improvement.
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